The purpose of this lab is to assemble and operate a paper chromatography apparatus, to study the meaning and significance of Rf values, and to identify the components of mixtures by means od Rf values.
For Part I, we set up the paper chromatography and prepare for the experiment. For Part II, we started with the blue dye on the chromatography paper. After 20 minutes, we are able to identify the solvent front and solute front and measure their distance to determine the Rf value. Part III is a similar process with two different dyes, one green and one unknown. Our task is to identify the components of the mixtures. The class as a whole recorded Class Results for Rf values as a reference. Finally, for table 3, we did the comparison for components of the coloring. The result for the green dye was yellow and blue by obervation and using Rf values. The result for the unknown dye (brown) was yello, red and blue.
At the end of our lab, we had time to do our lab report. As for conclusion, answer the question how to identify the components of a mixture. We came up with the answer to find Rf value first and compare with the standard value to match up the correct component.
Here is a link demonstrating similar concept of Rf value and the experiment of paper chromatograhy!
http://www.youtube.com/watch?v=3ZP_E0eTmMU
Our homework
- finish 3B lab report
- review answers online
- Chapter1 and 2 test next class (everything we've done so far, except safety)
- Bring scientific calculator
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